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International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
Issue per Year : 12
Volume Published : 11
Issue Published : 118
Article Submitted : 21545
Article Published : 8519
Total Authors : 22414
Total Reviewer : 805
Total Countries : 159
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| Paper Title: | To determine sensitivity & specificity between microscopy and polymerase chain reaction methods in patients presenting with acute undifferentiated fever and clinically suspected of malaria |
| Authors Name: | Jatav Kailash , Shah Harshada , Singh Luxmi , k Ramanath , Chaudhary Devendra |
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IJRTI_183923
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| Published Paper Id: | IJRTI2208259 |
| Published In: | Volume 7 Issue 8, August-2022 |
| DOI: | |
| Abstract: | To overcome some of the limitations associated with microscopy, molecular assays that detect Plasmodium specific nucleic acid sequences are increasingly being used. Aim: The aim of the present study was to perform peripheral blood smear examination for malaria parasite by Leishman stain in patients presenting with acute undifferentiated fever and clinically suspected of malaria and to compare them with Real-Time Polymerase Chain Reaction (PCR) for diagnosis of malaria in clinically suspected patients considering microscopy as gold standard. Materials & Methods: After obtaining Institutional Ethics Committee permission, the present study was conducted and informed consent from all the participants of the study was taken. The sample size of the present study is 154. Results: Despite the fact that 64 samples were identified as P.Falciparum by microscopy, only 46 were confirmed by PCR; one was negative and the other was P.Vivax. However, three samples identified as P.Falciparum by PCR were not identified as P.Falciparum by microscopy. he results of PCR of negative samples detected by microscopy (n=90) as a diagnostic quality. Between the samples detected by microscopy18 (14.7%) were false negatives based on PCR. Conclusion: The present study also demonstrated a highly sensitive and specific molecular method for detection of low level parasitemia in a targeted high-risk population which may prove it cost effective too. |
| Keywords: | Polymerase Chain Reaction (PCR), Malaria |
| Cite Article: | "To determine sensitivity & specificity between microscopy and polymerase chain reaction methods in patients presenting with acute undifferentiated fever and clinically suspected of malaria", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2455-2631, Vol.7, Issue 8, page no.1616 - 1620, August-2022, Available :http://www.ijrti.org/papers/IJRTI2208259.pdf |
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2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2208259
Registration ID:183923
Published In: Volume 7 Issue 8, August-2022
DOI (Digital Object Identifier):
Page No: 1616 - 1620 Country: Indore, Madhya Pradesh, India Research Area: Medical Science Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2208259 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2208259 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
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