 |
International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
Scholarly open access journals, Peer-reviewed, and Refereed Journals, Impact factor 8.14 (Calculate by google scholar and Semantic Scholar | AI-Powered Research Tool) , Multidisciplinary, Monthly, Indexing in all major database & Metadata, Citation Generator, Digital Object Identifier(DOI)
|
Issue: December 2025
Volume 10 | Issue 12
Review Result and Publication of Paper within : 2-3 days
Click Here For more DetailsFor Authors
Forms / Download
Published Issue Details
Editorial Board
Other IMP Links
Facts & Figure
Impact Factor : 8.14
Issue per Year : 12
Volume Published : 10
Issue Published : 115
Article Submitted : 20022
Article Published : 8163
Total Authors : 21559
Total Reviewer : 776
Total Countries : 147
Indexing Partner
Licence
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
|
Published Paper Details
|
|
| Paper Title: | Bioanalytical method development and validation of LC-ESI-MS/MS for estimation of Terazosin Hydrochloride Dihydrate in K3EDTA human plasma |
| Authors Name: | Patel Megha sunilbhai , Dr. Tulsidas Misra , Mr. Gajendra Solanki , Dr. Alisha Patel |
| Download E-Certificate: | Download |
| Author Reg. ID: |
IJRTI_189684
|
| Published Paper Id: | IJRTI2404134 |
| Published In: | Volume 9 Issue 4, April-2024 |
| DOI: | |
| Abstract: | To develop and validate a Bioanalytical method LC-ESI-MS/MS for estimation terazosin hydrochloride dihydrate in K3EDTA human plasma. A simple, rapid and sensitive isocratic reverse - phase liquid Chromatography - tandem mass spectrometric method equipped with Turbo Ion Spray (TIS) source, operating in the Positive ion and Multiple Reaction Monitoring (MRM) mode was developed to quantify terazosin hydrochloride dihydrate in human plasma. Liquid Liquid extraction (LLE) method was followed to extract analyte and ISTD from human plasma. For separation Mobile Phase Composition was 0.01% formic acid in 10 mM ammonium formate in water: Methanol (50:50% v/v) by using of column Betasil C8,150×4.6mm, 5µm. The drug and ISTD retained at about 2.6 minute with total run time 5 minute. After optimization, the Bio-analytical method was validated according to ICH, USFDA and EMA guidelines for Bio-analysis. Sample preparation was performed using Liquid Liquid extraction (LLE) and this method yielded clean extracts with recovery of 91.16 and 100.24 % for Drug and ISTD respectively. The response of the LC-MS/MS method for analyte was linear over the dynamic range of 0.400 – 400.000 ng/ml with regression coefficient r2 were found in range of 0.9962 to 0.9996. The Accuracy was within the accepted limit of ± 20% at lower limit of quantification (LLOQ) and ± 15 % at all the other concentrations in the linear range. A simple, precise and sensitive isocratic reverse-phase liquid chromatographic tandem mass spectrometric method was developed to quantify Terazosin Hydrochloride Dihydrate in KEDTA human plasma and can be successfully applied for BA/BE, toxicokinetic and pharmacokinetic studies in human subjects. |
| Keywords: | LC-ESI-MS/MS, Terazosin Hydrochloride Dihydrate, liquid liquid extraction, plasma, BA/BE studies |
| Cite Article: | "Bioanalytical method development and validation of LC-ESI-MS/MS for estimation of Terazosin Hydrochloride Dihydrate in K3EDTA human plasma", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.9, Issue 4, page no.987 - 994, April-2024, Available :http://www.ijrti.org/papers/IJRTI2404134.pdf |
| Downloads: | 000205128 |
| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
| Publication Details: |
Published Paper ID: IJRTI2404134
Registration ID:189684
Published In: Volume 9 Issue 4, April-2024
DOI (Digital Object Identifier):
Page No: 987 - 994 Country: vapi,valsad, Gujrat, India Research Area: Pharmacy Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2404134 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2404134 |
| Share Article: | |
|
Click Here to Download This Article |
|
| Article Preview | |
|
|
|
Major Indexing from www.ijrti.org
| Google Scholar | ResearcherID Thomson Reuters | Mendeley : reference manager | Academia.edu |
| arXiv.org : cornell university library | Research Gate | CiteSeerX | DOAJ : Directory of Open Access Journals |
| DRJI | Index Copernicus International | Scribd | DocStoc |
ISSN Details
 |
 |
|
ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
DOI (A digital object identifier)
| Providing A digital object identifier by DOI.ONE How to Get DOI? |
Conference
Open Access License Policy
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License
Important Details
Join RMS/Earn 300
WhatsApp
Click Here
Click Here
Indexing Partner |
|||
| Copyright © 2025 - All Rights Reserved - IJRTI | |||