ISSN2456-3315
Impact Factor10.57
UGC CAREApproved
Low Cost₹599 / $45
CrossRefDOI
ESTD2016
Peer ReviewTransparent
AccessOpen
Submission: Always Open
Paper Title
ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC
Authors
NIVETHA .R , NALINI C.N , VIJAYAGEETHA .R
Keywords
Keywords: HPLC, Alfacalcidol, ICH guidelines. Method Validation
Abstract
ABSTRACT :
The analytical method validation was simple, efficient, and less time-consuming for simultaneous estimation of alpha-calcidol capsules by HPLC. Chromatographic separation was accomplished in the isocratic mode. Mix 920 volumes of n-Hexane, 40 volumes of IPA, 40 volumes of tetrahydrofuran, and 2 volumes of acetic acid (920:40:40:2) in a 1000-mL beaker and mix well, then sonicate for 5 minutes as the mobile phase by using the column Thermo Scientific silica, 250 x 4.6 mm, 3µm, or equivalent as the stationary phase with a flow rate of 2.0 ml/min using an UV detector maintained at 265 nm. The reported periods of retention time of alfacalcidol were found to be 9.4 minutes. As per ICH guidelines, this procedure has been validated for specificity, linearity and range, accuracy, system precision, method precision, intermediate precision, robustness, solution stability, and filter integrity.
Full Paper, Downloads & Preview
How To Cite
Choose the style your journal or department asks for, then copy it. Every version below is generated from this paper's own record.
IJRTI — journal style
"ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC", IJRTI - International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2456-3315, Vol.9, Issue 6, page no.447 - 459, June-2024, Available :https://ijrti.org/papers/IJRTI2406063.pdf
APA — 7th edition
.R, N., C.N, N., & .R, V. (2024). ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC. International Journal for Research Trends and Innovation, 9(6), 447 - 459. https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063
MLA — 9th edition
.R, NIVETHA, et al. "ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC." International Journal for Research Trends and Innovation, vol. 9, no. 6, 2024, pp. 447 - 459, https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063.
Chicago — 17th, bibliography
.R, NIVETHA, NALINI C.N, and VIJAYAGEETHA .R. "ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC." International Journal for Research Trends and Innovation 9, no. 6 (2024): 447 - 459. https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063.
Harvard — author–date
.R, N., C.N, N. and .R, V. (2024) 'ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC', International Journal for Research Trends and Innovation, 9(6), pp. 447 - 459. Available at: https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063
IEEE — numbered reference
N. .R, N. C.N and V. .R, "ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC," IJRTI, vol. 9, no. 6, pp. 447 - 459, Jun. 2024.
Vancouver — biomedical
.R N, C.N N, .R V. ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC. IJRTI. 2024 Jun;9(6):447 - 459.
AMA — 11th edition
.R N, C.N N, .R V. ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC. IJRTI. 2024;9(6):447 - 459. Accessed at https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063
BibTeX — Zotero, Mendeley, LaTeX
@article{IJRTI2406063,
author = {NIVETHA .R and NALINI C.N and VIJAYAGEETHA .R},
title = {ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC},
journal = {International Journal for Research Trends and Innovation},
volume = {9},
number = {6},
pages = {447 - 459},
year = {2024},
month = {June},
issn = {2456-3315},
url = {https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - .R, NIVETHA
AU - C.N, NALINI
AU - .R, VIJAYAGEETHA
TI - ANALYTICAL METHOD VALIDATION FOR IDENTIFICATION AND ASSAY OF ALFACALCIDOL IN ALFACALCIDOL SOFT GELATIN CAPSULES BY HPLC
T2 - International Journal for Research Trends and Innovation
JA - IJRTI
VL - 9
IS - 6
PY - 2024
SN - 2456-3315
UR - https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063
SP - 447
EP - 459
ER -
Issue
Volume 9 Issue 6,
June-2024
Pages : 447 - 459
Other Publication Details
Paper Reg. ID
IJRTI_190061
Published Paper ID
IJRTI2406063
Downloads
205,663
Research Area
Pharmacy
Country
CHENNAI, TAMILNADU, INDIA
Published
June 2024
Published Paper PDF
https://ijrti.org/papers/IJRTI2406063.pdf
Published Paper URL
https://ijrti.org/viewpaperforall.php?paper=IJRTI2406063
About Publisher
International Journal for Research Trends and Innovation
Published by IJRTI (JW Publication)
2456-3315
ISSN
10.57
Impact Factor
2016
ESTD Year
Open
Access
Impact Factor 10.57 calculated by Google Scholar and Semantic Scholar.
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 10.57 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator
UGC CARE Approved Journal — Transparent Peer-reviewed journal aligned with UGC’s 2025 suggestive parameters, with CrossRef DOI registration and Scopus Standard metadata on every published paper.
Licence
© 2024 — Authors hold the copyright of this article.
This work is licensed under a
Creative Commons Attribution 4.0 International License
and The Open Definition.
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (CC BY 4.0),
which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Disclaimer:
The content, data and findings in this article are based on the authors’ research and have been
peer-reviewed for academic purposes only. Readers are advised to verify all information before practical
or commercial use. The journal and its editorial board are not liable for any errors, losses or
consequences arising from its use.
Declarations
Funding
No external funding was received for this study.
Conflict of Interest
The authors declare that they have no conflict of interest.
Acknowledgements
The authors would like to thank the reviewers and the editorial board of International Journal for Research Trends and Innovation for their careful reading and constructive comments, and all colleagues who supported the preparation of this manuscript.
