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Paper Title
A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants.
Authors
Sawalkar A.S. , Kadam R.M.
Keywords
HPTLC; qualitative; Rf value; marker compound; medicinal plants.
Abstract
Medicinal plants are very useful for farmers because they have bioactive compounds which show antifungal and antibacterial activities against plant pathogens. Medicinal plants have antifungal, antibacterial and anti-inflammation activities. The present study involves fifteen different medicinal plants locally available in Osmanabad and Beed district of Maharashtra.
The aim of the present work was to develop a high-performance thin-layer chromatography (HPTLC) densitometric analysis technique using the different solvent systems for selected fifteen medicinal plants, which has been developed and validated for simultaneous quantification of six marker compounds (quercetin, rutin, gallic acid, ascorbic acid ,curcumin and beta sitosterol) in plant extracts. The analysis was performed on HPTLC precoated silica gel 60F254 plates with as the mobile phase (distance of 6.5 cm). Densitometric detections of marker compounds were performed at 254 and 366 nm respectively. The results of HPTLC analysis of these medicinal showed that Curcumin (Rf value 0.75) present in Curcuma amada, Beta sitosterol (Rf value 0.64) estimated in Bauhinia variegata, Tinospora cordifolia, Eclipta alba, Moringa oleifera, Cassia tora, Citrus limon. Ascorbic acid (Rf value 0.23) is exactly match with Aegle marmelos. Quercetin (Rf value 0.78) estimated in Withania somenifera, Aloe vera, Psoralea cordifolia, Cassia agustifolia. Gallic acid (Rf value 0.51) in Azadirachta indica, Phyllanthus emblica and Punica granatum. Rutin absent in Phyllanthus emblica and Punica granatum. The HPTLC technique is very important commercially and has great interest in pharmaceutical companies for the production of new drugs for curing various diseases. It is expected that the important phytochemical properties recognized by our study in the indigenous medicinal plants of Osmanabad and Beed will be very useful in antifungal, antibacterial activities against plant pathogens for controlling various diseases of crop plants.
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IJRTI — journal style
"A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants.", IJRTI - International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2456-3315, Vol.9, Issue 1, page no.103 - 120, January-2024, Available :https://ijrti.org/papers/IJRTI2401019.pdf
APA — 7th edition
A.S., S., & R.M., K. (2024). A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants. International Journal for Research Trends and Innovation, 9(1), 103 - 120. https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019
MLA — 9th edition
A.S., Sawalkar, and Kadam R.M. "A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants." International Journal for Research Trends and Innovation, vol. 9, no. 1, 2024, pp. 103 - 120, https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019.
Chicago — 17th, bibliography
A.S., Sawalkar, and Kadam R.M. "A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants." International Journal for Research Trends and Innovation 9, no. 1 (2024): 103 - 120. https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019.
Harvard — author–date
A.S., S. and R.M., K. (2024) 'A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants', International Journal for Research Trends and Innovation, 9(1), pp. 103 - 120. Available at: https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019
IEEE — numbered reference
S. A.S. and K. R.M., "A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants," IJRTI, vol. 9, no. 1, pp. 103 - 120, Jan. 2024.
Vancouver — biomedical
A.S. S, R.M. K. A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants. IJRTI. 2024 Jan;9(1):103 - 120.
AMA — 11th edition
A.S. S, R.M. K. A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants. IJRTI. 2024;9(1):103 - 120. Accessed at https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019
BibTeX — Zotero, Mendeley, LaTeX
@article{IJRTI2401019,
author = {Sawalkar A.S. and Kadam R.M.},
title = {A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants.},
journal = {International Journal for Research Trends and Innovation},
volume = {9},
number = {1},
pages = {103 - 120},
year = {2024},
month = {January},
issn = {2456-3315},
url = {https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019}
}
RIS — EndNote, RefWorks
TY - JOUR
AU - A.S., Sawalkar
AU - R.M., Kadam
TI - A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants.
T2 - International Journal for Research Trends and Innovation
JA - IJRTI
VL - 9
IS - 1
PY - 2024
SN - 2456-3315
UR - https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019
SP - 103
EP - 120
ER -
Issue
Volume 9 Issue 1,
January-2024
Pages : 103 - 120
Other Publication Details
Paper Reg. ID
IJRTI_188838
Published Paper ID
IJRTI2401019
Downloads
205,621
Research Area
Life SciencesÂ
Country
Ambajogai, Maharashtra, India
Published
January 2024
Published Paper PDF
https://ijrti.org/papers/IJRTI2401019.pdf
Published Paper URL
https://ijrti.org/viewpaperforall.php?paper=IJRTI2401019
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International Journal for Research Trends and Innovation
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2456-3315
ISSN
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Funding
No external funding was received for this study.
Conflict of Interest
The authors declare that they have no conflict of interest.
Acknowledgements
The authors would like to thank the reviewers and the editorial board of International Journal for Research Trends and Innovation for their careful reading and constructive comments, and all colleagues who supported the preparation of this manuscript.
