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International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants. |
| Authors Name: | Sawalkar A.S. , Kadam R.M. |
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IJRTI_188838
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| Published Paper Id: | IJRTI2401019 |
| Published In: | Volume 9 Issue 1, January-2024 |
| DOI: | |
| Abstract: | Medicinal plants are very useful for farmers because they have bioactive compounds which show antifungal and antibacterial activities against plant pathogens. Medicinal plants have antifungal, antibacterial and anti-inflammation activities. The present study involves fifteen different medicinal plants locally available in Osmanabad and Beed district of Maharashtra. The aim of the present work was to develop a high-performance thin-layer chromatography (HPTLC) densitometric analysis technique using the different solvent systems for selected fifteen medicinal plants, which has been developed and validated for simultaneous quantification of six marker compounds (quercetin, rutin, gallic acid, ascorbic acid ,curcumin and beta sitosterol) in plant extracts. The analysis was performed on HPTLC precoated silica gel 60F254 plates with as the mobile phase (distance of 6.5 cm). Densitometric detections of marker compounds were performed at 254 and 366 nm respectively. The results of HPTLC analysis of these medicinal showed that Curcumin (Rf value 0.75) present in Curcuma amada, Beta sitosterol (Rf value 0.64) estimated in Bauhinia variegata, Tinospora cordifolia, Eclipta alba, Moringa oleifera, Cassia tora, Citrus limon. Ascorbic acid (Rf value 0.23) is exactly match with Aegle marmelos. Quercetin (Rf value 0.78) estimated in Withania somenifera, Aloe vera, Psoralea cordifolia, Cassia agustifolia. Gallic acid (Rf value 0.51) in Azadirachta indica, Phyllanthus emblica and Punica granatum. Rutin absent in Phyllanthus emblica and Punica granatum. The HPTLC technique is very important commercially and has great interest in pharmaceutical companies for the production of new drugs for curing various diseases. It is expected that the important phytochemical properties recognized by our study in the indigenous medicinal plants of Osmanabad and Beed will be very useful in antifungal, antibacterial activities against plant pathogens for controlling various diseases of crop plants. |
| Keywords: | HPTLC; qualitative; Rf value; marker compound; medicinal plants. |
| Cite Article: | "A High Performance Thin Layer Chromatographic Method Using a Design of Experiment Approach For Estimation of Phytochemical in Extracts of Selected Medicinal Plants.", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.9, Issue 1, page no.103 - 120, January-2024, Available :http://www.ijrti.org/papers/IJRTI2401019.pdf |
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2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
| Publication Details: |
Published Paper ID: IJRTI2401019
Registration ID:188838
Published In: Volume 9 Issue 1, January-2024
DOI (Digital Object Identifier):
Page No: 103 - 120 Country: Ambajogai, Maharashtra, India Research Area: Life Sciences Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2401019 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2401019 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
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