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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | Detection of Helicobacter pylori antigen in stool by Enzyme Linked Immunosorbent Assay and comparison with conventional methods. |
| Authors Name: | Dr. Krithikaa Sekar |
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| Author Reg. ID: |
IJRTI_184722
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| Published Paper Id: | IJRTI2211088 |
| Published In: | Volume 7 Issue 11, November-2022 |
| DOI: | |
| Abstract: | Background: The H. pylori have been associated with gastritis, peptic ulcers, gastric adenocarcinoma, and gastric mucosa-associated lymphoid type (MALT) B-cell lymphomas. In the presence of upper gastrointestinal bleeding, the diagnosis of H pylori infection may be compromised. Therefore, it is suggested that noninvasive methods, such as serology, be used to identify H pylori infection in these patients. The diagnostic role of Helicobacter pylori stool antigen enzyme immunoassay (HpSA) and simple H pylori antigen cassette test in patients with upper gastrointestinal bleeding remains unclear. Hence we proposed to conduct this study to assess the efficacy of ELISA with conventional methods in detecting H. pylori infection. Materials and Methods: The details of complete history, clinical feature of the patients subjected to endoscopy were obtained. Preinvasive procedure preparation for oesophago gastro duodenoscopy was performed as per norms. Stool sample is obtained from the patient on the day of endoscopy. Three biopsy sample were collected, two from the gastric antrum and one from the corpus. One biopsy sample from the antrum was used for performing Rapid urease test and the other two samples were placed in 10% formalin and sent for histopathological examination. Results: 57% of gastritis patients and 56% of duodenitis patients are positive for HpSAg by Enzyme Linked Immunosorbent Assay. Out of the total 120 samples, 30 were positive by both ELISA and gold standard tests. 4 were positive by gold standard but negative by ELISA. 16 were positive by ELSA but negative by gold standard tests. 70 were negative by both ELISA and gold standard tests. Comparing ELISA with the gold standard, the sensitivity was 100%, specificity was 77%, Positive predictive value was 52% and Negative Predictive value was 100%. Conclusion: Detection of H. pylori antigens using HpSA shows a high sensitivity and specificity and might be useful for non-invasive diagnosis of H. pylori infection in children and adult patients, hence recommended for the diagnosis of H.pylori infection. |
| Keywords: | H.pylori, UBT, Gastritis, Duodenitis, ELISA, sensitivity, specificity, PPV, NPV, Gold standard |
| Cite Article: | "Detection of Helicobacter pylori antigen in stool by Enzyme Linked Immunosorbent Assay and comparison with conventional methods.", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.7, Issue 11, page no.616 - 628, November-2022, Available :http://www.ijrti.org/papers/IJRTI2211088.pdf |
| Downloads: | 000205182 |
| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2211088
Registration ID:184722
Published In: Volume 7 Issue 11, November-2022
DOI (Digital Object Identifier):
Page No: 616 - 628 Country: CHENNAI, TAMIL NADU, India Research Area: Medical Science Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2211088 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2211088 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
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