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International Journal for Research Trends and Innovation
International Peer Reviewed & Refereed Journals, Open Access Journal
ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
Issue per Year : 12
Volume Published : 11
Issue Published : 118
Article Submitted : 22135
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Total Authors : 22851
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| Paper Title: | Optimization Of Process Parameters For Esterase Production By Biofilm-forming Estuarine Isolate BSE01 Sourced From Sundarbans In A Novel Enhanced Surface Area Conico-Cylindrical Flask |
| Authors Name: | Dr Sreyashi Sarkar |
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IJRTI_189291
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| Published Paper Id: | IJRTI2403017 |
| Published In: | Volume 9 Issue 3, March-2024 |
| DOI: | |
| Abstract: | Esterase is produced by biofilm-forming estuarine isolate, BSE01, a Bacillus sp., in a novel polymethylmethacrylate enhanced surface area conico-cylindrical flask that has significantly higher surface area with a property favorable to surface attachment. Then, the process operating parameters are studied using Response Surface Methodology wherein pH, temperature and rotational speed are considered as factors. Each of the three parameters are studied at three levels viz. high, medium and low –8, 7 and 6 for pH; 42°C, 37°C, 32°C for temperature and 160 rpm, 140 rpm and 120 rpm for rotational speed having 100 mL total volume and 96h of incubation period. The peak esterase activity (PEA) is determined and considered as a response. The optimum process conditions for esterase production at 72h of incubation period are determined (pH 7, 37°C, 140 rpm). The model predicted the PEA value of 2229 U/mL at optimal value of process parameters (pH 7, 37.5°C, 140 rpm). The experiment performed with the same optimum conditions gave PEA value i.e. 2310 U/mL with 3.63 % error. |
| Keywords: | Esterase, production, estuarine, biofilm-forming, process, parameter, optimization |
| Cite Article: | "Optimization Of Process Parameters For Esterase Production By Biofilm-forming Estuarine Isolate BSE01 Sourced From Sundarbans In A Novel Enhanced Surface Area Conico-Cylindrical Flask", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2456-3315, Vol.9, Issue 3, page no.113 - 118, March-2024, Available :http://www.ijrti.org/papers/IJRTI2403017.pdf |
| Downloads: | 000205336 |
| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2403017
Registration ID:189291
Published In: Volume 9 Issue 3, March-2024
DOI (Digital Object Identifier):
Page No: 113 - 118 Country: KOLKATA, WESTBENGAL, India Research Area: Science & Technology Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2403017 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2403017 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
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