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Paper Title:
Process Parameters Optimization For Protease Production By Biofilm-forming Chicken Meat Isolate Vergibacillus pantothenticus (MTCC 6729) In A Novel Enhanced Surface Area Conico-Cylindrical Flask
Protease is produced by biofilm-forming chicken meat isolate, Vergibacillus pantothenticus (MTCC 6729) in a novel polymethylmethacrylate enhanced surface area conico-cylindrical flask that has increased surface area with a favorable surface attachment property. Then, the process operating parameters are studied using Response Surface Methodology wherein pH, temperature and rotational speed are considered as factors. Each of the three parameters are studied at three levels viz. high, medium and low –10, 9 and 8 for pH; 45°C, 40°C, 35°C for temperature and 160 rpm, 140 rpm and 120 rpm for rotational speed having 100 mL total volume and 96h of incubation period. The peak protease activity (PPA) is determined and considered as a response. The optimum process conditions for protease production at 48h of incubation period are determined (pH 9, 40°C, 140 rpm). The model predicted the PPA value of 16.36 U/mL at optimal value of process parameters (pH 8.8, 40°C, 140 rpm). The experiment performed with the same optimum conditions gave PPA value i.e. 17.2 U/mL with 4.88 % error.
"Process Parameters Optimization For Protease Production By Biofilm-forming Chicken Meat Isolate Vergibacillus pantothenticus (MTCC 6729) In A Novel Enhanced Surface Area Conico-Cylindrical Flask", International Journal for Research Trends and Innovation (www.ijrti.org), ISSN:2455-2631, Vol.9, Issue 4, page no.142 - 147, April-2024, Available :http://www.ijrti.org/papers/IJRTI2404021.pdf
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ISSN:
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016
An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator