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International Journal for Research Trends and Innovation
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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | A Review on Separation of Phytoconstituents Present in Aqueous Extract of Azadirachta Indica bt TLC/HPLC/Column Chromatography Method |
| Authors Name: | Dr. Balkrishna Tiwari , Dr. Yogesh Thorat , Prachet Bagewadikar , Shruti Narayankar , Pornima Parshetti |
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IJRTI_189945
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| Published Paper Id: | IJRTI2405086 |
| Published In: | Volume 9 Issue 5, May-2024 |
| DOI: | |
| Abstract: | Azadirachta indica. L(Meliaceae) is smart in distribution and generally used in several native medical practices. In India, varied vegetative parts of A. indica, which includes leaves, flowers, fruits, seeds, and bark are extensively used in Indian traditional drug for treating varied human conditions and diseases. It’s also called as storage of phytochemicals. The chemical composition is relatively complex. Numerous medicinal plants studied for their phytochemical contents. Azadirachta indica is one of these plants. This research, primarily aimed to carry out a primary phytochemical screening to determine the major classes of bioactive composites presented in Azadirachta indica dry leaves. Numerous solvent (Methanol, Ethanol, Ethyl acetate, Acetone, acetic acid and Distilled water) were used to determine the stylish solvent that can be used for extraction and to perform Thin Layer Chromatography profiling of all successional extracts.TLC was carried out in Silica gel plates using mobile phase protocol, Chloroform Ethanol Methanol (111) and using six mentioned solvents. Thin Layer Chromatographic studies of the Azadirachta indica leaf extract (Methanol, Ethanol, Ethyl acetate, Acetone, Acetic acid and Distilled water) constituted different coloured phytochemical composites with different Rf values. Methanol extract is the stylish which shows seven bands with Rf lower than 1 for 24 hrs. After seven days among all the extracts, methanol extract was set up to contain the outside number of bioactive composites which showed seven bands with Rf lower than 1. High Performance Liquid Chromatography(HPLC) point of the constituents of acetone extracts of A. indica was farther investigated. Highest change yield (10.20) was attained for acetone extract of A. indica. Varying ingredients glycosides, protein, anthraquinone, flavonoid, tannin and terpenoid were identify. An array of mixes was separated at different peak heights corresponding to concentration of the mixes Myricetin was detected in topmost chance (39.6) in acetone extracts of A.indica while alpha funebren detected in lowest chance(1.5).Column chromatographic studies of methanolic extract using the solvent system Chloroform ethanol methanol, shows the better separation with methanol solvent. From these research it concluded that the methanol extract for Azadirachta indica leaves contain a advanced content of bioactive composites, which can be used for farther research on this plant. |
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| Cite Article: | "A Review on Separation of Phytoconstituents Present in Aqueous Extract of Azadirachta Indica bt TLC/HPLC/Column Chromatography Method", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.9, Issue 5, page no.622 - 629, May-2024, Available :http://www.ijrti.org/papers/IJRTI2405086.pdf |
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| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2405086
Registration ID:189945
Published In: Volume 9 Issue 5, May-2024
DOI (Digital Object Identifier):
Page No: 622 - 629 Country: Solapur, Maharashtra, India Research Area: Pharmacy Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2405086 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2405086 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
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