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International Journal for Research Trends and Innovation
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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
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| Paper Title: | A validated high‑performance thin‑layer chromatography method for the simultaneous estimation of Jatrorrhizine, Palmatine and Magnoflorine from Stephania glabra. |
| Authors Name: | Prerana I Parmar , Mamta B. Shah , Krupa V. Gadhvi |
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IJRTI_200383
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| Published Paper Id: | IJRTI2501056 |
| Published In: | Volume 10 Issue 1, January-2025 |
| DOI: | |
| Abstract: | Stephania glabra (Roxb.) Miers, a climbing shrub belonging to the family Menispermaceae, is well-recognized in India and distributed across tropical and subtropical regions, particularly in the Himalayan foothills at altitudes up to 2200 m. Traditionally, this plant is highly valued in Ayurveda and other medicinal systems for treating a wide range of ailments, including diarrhea, pyrexia, tuberculosis, dyspepsia, urinary tract infections, abdominal disorders, asthma, dysmenorrhea, wound healing, and headaches. Pharmacological studies reveal that its activity is primarily attributed to its alkaloids, such as stepharine, gindarine, magnoflorine, jatrorrhizine, palmatine, and others, which exhibit antimicrobial, antiplasmodial, antifungal, and neuroprotective properties. This study aimed to validate a high-performance thin-layer chromatography (HPTLC) method for the simultaneous estimation of three major alkaloids—Palmatine, Magnoflorine, and Jatrorrhizine—in Stephania glabra tuber Ethanol extracts of the dried tubers were analyzed on pre-coated silica gel plates using ethyl acetate‒formic acid‒glacial acetic acid‒water (100:11:11:26, v/v) as the mobile phase. The plates were scanned at 254 nm and 366 nm using a TLC Scanner with vision CATS software. The method was validated for linearity, precision, accuracy, robustness, and specificity per ICH guidelines. Calibration plots for Palmatine (1000–6000 ng/spot), Magnoflorine (500–3000 ng/spot), and Jatrorrhizine (280–1680 ng/spot) demonstrated high linearity (r ≥ 0.99). The method showed excellent reproducibility with intra- and inter-day precision, and peak purity confirmed its specificity. The developed method offers a sensitive, accurate, and economical approach for the quantitative and qualitative analysis of Stephania glabra tuber. |
| Keywords: | Stephania glabra, Alkaloids, Palmatine, Magnoflorine, and Jatrorrhizine |
| Cite Article: | "A validated high‑performance thin‑layer chromatography method for the simultaneous estimation of Jatrorrhizine, Palmatine and Magnoflorine from Stephania glabra.", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.10, Issue 1, page no.a440-a447, January-2025, Available :http://www.ijrti.org/papers/IJRTI2501056.pdf |
| Downloads: | 000571 |
| ISSN: |
2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
| Publication Details: |
Published Paper ID: IJRTI2501056
Registration ID:200383
Published In: Volume 10 Issue 1, January-2025
DOI (Digital Object Identifier):
Page No: a440-a447 Country: ahmedabad, Gujarat, India Research Area: Pharmacy Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2501056 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2501056 |
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ISSN: 2456-3315
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