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International Journal for Research Trends and Innovation
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ISSN Approved Journal No: 2456-3315 | Impact factor: 8.14 | ESTD Year: 2016
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Impact Factor : 8.14
Issue per Year : 12
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| Paper Title: | Detection and Characterization of Nontuberculous Mycobacteria from selected water sources in the Central Region of Ghana |
| Authors Name: | Kofi D. Bedzra , Yvonne E. Quist , Oti K. Gyamfi , Daniel Asante , Foster Kyei, Emmanuel Plas Otwe |
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IJRTI_200625
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| Published Paper Id: | IJRTI2503007 |
| Published In: | Volume 10 Issue 3, March-2025 |
| DOI: | |
| Abstract: | Background: Nontuberculous mycobacteria (NTMs) are common saprophytes found in environmental niches. These mycobacteria species are not classified as members of the Mycobacterium tuberculosis complex (MTBC). There are very few data on the isolation and identification of NTM from water sources in Ghana. The main objective of this study was to isolate and classify NTM infections occurring in various sources of drinking water that are frequently used by inhabitants of the Central Region of Ghana. Methods: In total, 45 water samples were collected from river bodies, Ghana Water Company Limited treatment plants, community taps, wells and boreholes. Conventional acid-fast staining, culture on Lowestein-Jensen (L-J) slants, the Capilia® TB-Neo assay, PCR and PCR-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis were also performed. The 360 bp fragment of the rpoB gene was amplified for isolates characterised as NTM, and the products were individually subjected to digestion with the restriction endonucleases MspI and HaeIII. Results: Thirty-one (31; 68.9%) of the isolates obtained from the samples were confirmed by the Capilia TB-Neo assay to be NTM. The rpoB gene was detected in seventeen (17; 54.8%) of the samples. PCR-restriction fragment length polymorphism analysis revealed Mycobacterium celatum, Mycobacterium gordonae type II, Mycobacterium simae, Mycobacterium genavense, Mycobacterium kansasii type V, Mycobacterium kansasii type I, and Mycobacterium phlei in the water samples. Conclusion: Hopefully, the findings from this study will add to the baseline information on the occurrence and characterisation of NTMs in various water sources (in the Central Region of Ghana). |
| Keywords: | NTM; Public Health; Drinking Water; Mycobacteria, PCR-RFLP |
| Cite Article: | "Detection and Characterization of Nontuberculous Mycobacteria from selected water sources in the Central Region of Ghana", International Journal of Science & Engineering Development Research (www.ijrti.org), ISSN:2455-2631, Vol.10, Issue 3, page no.a35-a51, March-2025, Available :http://www.ijrti.org/papers/IJRTI2503007.pdf |
| Downloads: | 000394 |
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2456-3315 | IMPACT FACTOR: 8.14 Calculated By Google Scholar| ESTD YEAR: 2016 An International Scholarly Open Access Journal, Peer-Reviewed, Refereed Journal Impact Factor 8.14 Calculate by Google Scholar and Semantic Scholar | AI-Powered Research Tool, Multidisciplinary, Monthly, Multilanguage Journal Indexing in All Major Database & Metadata, Citation Generator |
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Published Paper ID: IJRTI2503007
Registration ID:200625
Published In: Volume 10 Issue 3, March-2025
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Page No: a35-a51 Country: Accra, Greater Accra, Ghana Research Area: Biological Science Publisher : IJ Publication Published Paper URL : https://www.ijrti.org/viewpaperforall?paper=IJRTI2503007 Published Paper PDF: https://www.ijrti.org/papers/IJRTI2503007 |
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ISSN: 2456-3315
Impact Factor: 8.14 and ISSN APPROVED,
Journal Starting Year (ESTD) : 2016
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